Monoclonal Antibody to Vimentin (VIM) Homo sapiens (Human) Monoclonal antibody

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB040Hu01-Recombinant Vimentin (VIM)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Vimentin (VIM) Packages (Simulation)
  • Monoclonal Antibody to Vimentin (VIM) Packages (Simulation)
  • Monoclonal Antibody to Vimentin (VIM) DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 10µg/ml Mouse Anti-Human VIM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • MAB040Hu21.jpg WB Image
  • Monoclonal Antibody to Vimentin (VIM) Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Canine Heart lysate; Lane3: Bovine Heart lysate; Lane4: Caprine Heart lysate; Lane5: Gallus Heart lysate; Lane6: Equine Heart lysate
    Primary Ab: 0.15µg/ml Mouse Anti-Human VIM Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against VIM. It has been selected for its ability to recognize VIM in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 1-20µg/mL
Immunocytochemistry: 1-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

Citations

  • Aberrant Vimentin Methylation Is Characteristic of Breast Cancerpubmed:22315367
  • Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates.pubmed:27898103
  • HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperoninPubmed:29477555
  • Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathyPubmed: 31511328
  • Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allograftsPubmed: 31729155
  • Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development?Pubmed: 32088750
  • ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer33566221
  • Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence33758996
  • Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women
  • Advanced glycation end-products associate with podocytopathy in type II diabetic patients
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …

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