Monoclonal Antibody to Vitamin D Binding Protein (DBP) Homo sapiens (Human) Monoclonal antibody

GC; VDBG; VDBP; Group-Specific Component; Gc-globulin

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB810Hu02-Recombinant Vitamin D Binding Protein (DBP)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Vitamin D Binding Protein (DBP) Packages (Simulation)
  • Monoclonal Antibody to Vitamin D Binding Protein (DBP) Packages (Simulation)
  • Monoclonal Antibody to Vitamin D Binding Protein (DBP) DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10µg/ml Mouse Anti-Human DBP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Vitamin D Binding Protein (DBP) DAB staining on IHC-P;
    Sample: Human Liver Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human DBP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB810Hu21.jpg WB Image
  • Monoclonal Antibody to Vitamin D Binding Protein (DBP) Western Blot; Sample: Lane1: Human Serum; Lane2: Human Lung lysate; Lane3: Human Placenta lysate; Lane4: Human Plasma; Lane5: Rat Plasma
    Primary Ab: 3µg/ml Mouse Anti-Human DBP Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against DBP. It has been selected for its ability to recognize DBP in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patientsPubMed: 23101585
  • Increased Circulating Levels of Vitamin D Binding Protein in MS PatientsPubmed:25590278
  • Vitamin D-binding protein and free vitamin D concentrations in acromegalyPubMed: 26547217
  • Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
  • 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients.pubmed:28085957
  • Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
  • 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients10.1371/journal.pone.0170323
  • Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …Pubmed:30037068
  • Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic …Pubmed: 30553018
  • Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children?34013709
  • Leukocyte telomere length as a compensatory mechanism in vitamin D metabolismPubmed:35202418

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