Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Rattus norvegicus (Rat) Monoclonal antibody

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Packages (Simulation)
  • Monoclonal Antibody to Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against WNT3A. It has been selected for its ability to recognize WNT3A in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Wls promotes the proliferation of breast cancer cells via Wnt signalingPubmed:25801232
  • Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIsPubMed: 26209081
  • Gelatin device for the delivery of growth factors involved in endochondral ossificationpubmed:28380024
  • Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.pubmed:28754618
  • Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus MusclePubmed:30151071
  • Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast CancerDoi: 10.15369/sujms.30.359
  • Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular CarcinomaPubmed: 31737122
  • Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infectionPubmed: 32920199
  • An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties34823885

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