Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) Mus musculus (Mouse) Polyclonal antibody

11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short Chain Dehydrogenase/Reductase Family 26C,Member 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC268Mu01-Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.45mg/mL
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  • Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) Packages (Simulation)
  • Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) Packages (Simulation)
  • Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) Western Blot; Sample: Mouse Liver lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Mouse HSD11b1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HSD11b1. It has been selected for its ability to recognize HSD11b1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Uninephrectomy reduces 11β-hydroxysteroid dehydrogenase type 1 and type 2 concomitantly with an increase in blood pressure in rats PubMed: 22739210
  • Anti-Inflammatory Property ofPubMed: 26300946
  • Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serumand Bone in Rat Model of Glucocorticoid-Induced Osteoporosis.pubmed:27854305
  • A novel derivatives of thiazol-4 (5H)-one and their activity in the inhibition of 11β-hydroxysteroid dehydrogenase type 1Pubmed:29738970
  • An Evidence-Based Review: The Effects of Malaysian Traditional Herbs on Osteoporotic Rat Models
  • Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase InhibitorsDoi: BioMed Research International
  • Synthesis of the N‐methyl derivatives of 2‐aminothiazol‐4 (5H)‐one and their interactions with 11βHSD1‐molecular modeling and in vitro studiesPubmed: 31012543
  • Hepatoprotective Effects of Morinda citrifolia Leaf Extract on Ovariectomized Rats Fed with Thermoxidized Palm Oil Diet: Evidence at Histological and Ultrastructural …Pubmed: 31827717

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