Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)

ACT-A2; ACTSA; ACTVS; Asma; GIG46; Cell growth-inhibiting gene 46 protein

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 110.00 US$ 258.00 US$ 368.00 US$ 920.00 US$ 3,680.00
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Overview

Properties

Product No.PAB342Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryDevelopmental science Bone metabolism

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB342Hu01-Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Esophagus Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Western Blot; Sample: Rat Skeletal muscle lysate; Primary Ab: 5µg/ml Rabbit Anti-Human ACTa2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Skeletal muscle lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Rat Heart lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human ACTa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Line1: Human Lung lysate; Line2: Bovine Heart lysate; Line3: Caprine Liver lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human ACTA2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ACTa2. It has been selected for its ability to recognize ACTa2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-5µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat)

Giveaways

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Citations

Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaPubmed:27157496
Effects of repeated increasing doses of cisplatin as models of acute kidney injury and chronic kidney disease in ratsPubmed: 32936352
Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …34857049
NOX5 mediates the crosstalk between tumor cells and cancer‐associated fibroblasts via regulating cytokine network34459125