Polyclonal Antibody to Beta Actin (ACTB) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus) Polyclonal antibody

Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB340Mi01-Recombinant Beta Actin (ACTB)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.44mg/ml
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  • Polyclonal Antibody to Beta Actin (ACTB) Packages (Simulation)
  • Polyclonal Antibody to Beta Actin (ACTB) Packages (Simulation)
  • Polyclonal Antibody to Beta Actin (ACTB) Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
  • Polyclonal Antibody to Beta Actin (ACTB) DAB staining on IHC-P;
    Sample: Rat Cardiac Muscle Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Multi-species ACTB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Beta Actin (ACTB) DAB staining on IHC-P;
    Sample: Rat Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Multi-species ACTB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB340Mi01.jpg Figure. Western Blot; Sample: Recombinant ACTb.
  • Polyclonal Antibody to Beta Actin (ACTB) Figure. Western Blot; Sample: Lane1: Human HeLa Cells; Lane2: Human HepG2 Cells; Lane3: Human MCF-7 Cells; Lane4: Human BXPC-3 Cells; Lane5: Human A875 Cells; Lane6: Human A431 Cells; Lane7: Human Raji Cells; Lane8: Human RAW264.7 Cells; Lane9: Human PC-3 Cells; Lane10: Human SGC7901 Cells; Lane11: Human HL60 Cells; Lane12: Human A-375 Cells; Lane13: Human Jurkat Cells; Lane14: Human SKOV3 Cells.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ACTB. It has been selected for its ability to recognize ACTB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
  • Expression of chloride channels in mouse skin incisional wounds96
  • Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes
  • Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo MedicinePubmed: 31902915
  • Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
  • Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteriesPubmed: 32784207
  • Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring
  • Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells33261077
  • POLRMT mutations impair mitochondrial transcription causing neurological disease33602924
  • Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexusPubmed:34990649
  • Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn.Pubmed:35398498
  • Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer

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