Polyclonal Antibody to Amyloid Precursor Protein (APP) Homo sapiens (Human) Polyclonal antibody

AAA; ABeta; ABPP; AD1; APPI; CVAP; PN2; CTFgamma; Peptidase Nexin-II Alzheimer Disease; Peptidase Nexin-II; Amyloid Beta(A4)Precursor Protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB020Hu03-Recombinant Amyloid Precursor Protein (APP)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Amyloid Precursor Protein (APP) Packages (Simulation)
  • Polyclonal Antibody to Amyloid Precursor Protein (APP) Packages (Simulation)
  • Polyclonal Antibody to Amyloid Precursor Protein (APP) Western Blot; Samples: Lane1: SK-N-SH cell lysate; Lane2: U87MG cell lysate;
    Primary Ab: 0.3µg/ml Rabbit Anti-Human APP Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against APP. It has been selected for its ability to recognize APP in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Bilobalide regulates soluble amyloid precursor protein release via phosphatidyl inositol 3 kinase-dependent pathwayScienceDirect: S0197018611001641
  • Substitution of membrane cholesterol with β-sitosterol promotes nonamyloidogenic cleavage of endogenous amyloid precursor proteinPubmed: 23707801
  • PROOF COVER SHEET
  • (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探
  • Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
  • Probiotics ameliorates LPS induced neuroinflammation injury on Aβ 1–42, APP, γ-β secretase and BDNF levels in maternal gut microbiota and fetal …Pubmed:35312928
  • The positive impact of 12 weeks of dance and balance training on the circulating amyloid precursor protein and serotonin concentration as well as physical and …Pubmed:35217193

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