Polyclonal Antibody to Apolipoprotein B (APOB) Homo sapiens (Human) Polyclonal antibody

Apo-B; FLDB; Apo B-100; Apo B-48

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC003Hu02-Recombinant Apolipoprotein B (APOB)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Apolipoprotein B (APOB) Packages (Simulation)
  • Polyclonal Antibody to Apolipoprotein B (APOB) Packages (Simulation)
  • PAC003Hu02.jpg Figure. Western Blot; Sample: Recombinant APOB, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against APOB. It has been selected for its ability to recognize APOB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Group 1B phospholipase A2 deficiency protects against diet-induced hyperlipidemia in miceJlr: M019463
  • Elevated retinol binding protein 4 induces apolipoprotein B production and associates with hypertriglyceridemiaPubmed:25781360
  • Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolismPubMed: 26515253
  • Hypoascorbemia induces atherosclerosis and vascular deposition of lipoprotein (a) in transgenic micePubMed: 26064792
  • Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradationPubmed:27284008
  • COMPOSITIONS AND METHODS FOR EFFICACIOUS AND SAFE DELIVERY OF SIRNA USING SPECIFIC CHITOSAN-BASED NANOCOMPLEXESy2016:0152988.html
  • Hops (Humulus lupulus) Content in Beer Modulates Effects of Beer on the Liver After Acute Ingestion in Female Mice.pubmed:27659607

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