Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) Homo sapiens (Human) Polyclonal antibody

COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA350Hu02-Recombinant Collagen Type I Alpha 1 (COL1a1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) Packages (Simulation)
  • Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) Packages (Simulation)
  • Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) Used in DAB staining on fromalin fixed paraffin- embedded Kidney tissue
  • PAA350Hu02.jpg Figure. Western Blot; Sample: Recombinant COL1a1, Human.
  • Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) Figure. Western Blot; Sample: Lane1: Human Placenta Tissue; Lane2: Human Liver Tissue; Lane3: Porcine Skin Tissue.
  • Polyclonal Antibody to Collagen Type I Alpha 1 (COL1a1) Western Blot; Sample: Porcine Skin lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Human COL1a1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against COL1a1. It has been selected for its ability to recognize COL1a1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Simvastatin-loaded porous implant surfaces stimulate preosteoblasts differentiation: an in vitro studyScienceDirect: S1079210410004919
  • Osteoblast response to puerarin-loaded porous titanium surfaces: An in vitro studyWiley: source
  • Age effects on extracellular matrix production of vocal fold scar fibroblasts in ratsPubmed: 24077847
  • Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study Pubmed: 24495429
  • Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells.Pubmed: 24370188
  • Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propriaPubmed:25545625
  • Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-cultureUnivmed:Source
  • The vitamin D receptor agonist, calcipotriol, modulates fibrogenic pathways mitigating liver fibrosis in-vivo: An experimental study.pubmed:27477355
  • Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
  • Prevention of Diabetic Nephropathy by Modified Acidic Fibroblast Growth Factorpubmed:28768285
  • Effect of TGFβ1, TGFβ3 and keratinocyte conditioned media on functional characteristics of dermal fibroblasts derived from reparative (Balb/c) and …Pubmed:29637306
  • LOXL2, a copper-dependent monoamine oxidase, activates lung fibroblasts through the TGF-β/Smad pathwayPubmed: 30320382
  • Association between Plasma HMGB-1 and Silicosis: A Case-Control StudyPubmed: 30558126
  • Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detectionPubmed: 31706945
  • Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm
  • Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response
  • Inhibition of eEF2K synergizes with glutaminase inhibitors or 4EBP1 depletion to suppress growth of triple-negative breast cancer cells33911160
  • Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro ModelPubmed:35682677

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