Polyclonal Antibody to Complement Component 5 (C5) Homo sapiens (Human) Polyclonal antibody

CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB969Hu01-Recombinant Complement Component 5 (C5)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Complement Component 5 (C5) Packages (Simulation)
  • Polyclonal Antibody to Complement Component 5 (C5) Packages (Simulation)
  • Polyclonal Antibody to Complement Component 5 (C5) DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human C5 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 5 (C5) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human C5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 5 (C5) DAB staining on IHC-P;
    Samples: Human amygdalitis Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human C5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 5 (C5) DAB staining on IHC-P;
    Samples: Human liver cirrhosis Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human C5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 5 (C5) DAB staining on IHC-P;
    Sample: Human liver Cirrhosis Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human C5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 5 (C5) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human C5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Component 5 (C5) DAB staining on IHC-P;
    Sample: Human Stomach Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human C5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB969Hu01.jpg Western Blot; Sample: Lane1: Human Serum; Lane2: Human Plasma
    Primary Ab: 3µg/ml Rabbit Anti-Human C5 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against C5. It has been selected for its ability to recognize C5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
  • Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
  • Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expressionpubmed:29031586
  • iTRAQ‐Based Proteomic Analysis Reveals Protein Profile in Plasma from Children with AutismPubmed:29274201
  • Impaired insulin signaling in the B10.D2-Hc H2 H2-T18/oSnJ mouse model of complement factor 5 (C5) deficiencyPubmed: 31084499
  • Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancyPubmed: 31162828
  • C5aR deficiency attenuates the breast cancer development via the p38/p21 axisPubmed: 32669478

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