Polyclonal Antibody to Complement Factor B (CFB) Homo sapiens (Human) Polyclonal antibody

CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC011Hu04-Recombinant Complement Factor B (CFB)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Complement Factor B (CFB) Packages (Simulation)
  • Polyclonal Antibody to Complement Factor B (CFB) Packages (Simulation)
  • Polyclonal Antibody to Complement Factor B (CFB) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human CFB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Factor B (CFB) DAB staining on IHC-P;
    Sample: Human Liver Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human CFB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC011Hu04.jpg Western Blot; Sample: Human Serum
    Primary Ab: 1μg/ml Rabbit Anti-Human CFB Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CFB. It has been selected for its ability to recognize CFB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysisBioMed: 14712369
  • Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MSACS: pr300330z
  • Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
  • Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
  • Adipose tissue complement factor B promotes adipocyte maturation.pubmed:29137982
  • Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection
  • Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han populationPubmed: 31857673
  • Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis33367879
  • Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse33410734
  • Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role34766501
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889

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