Polyclonal Antibody to Complement Factor H (CFH)

CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 97.00 US$ 227.00 US$ 324.00 US$ 810.00 US$ 3,240.00
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Overview

Properties

Product No.PAA635Mu02
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryMetabolic pathway Infection immunity Immune molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA635Mu02-Recombinant Complement Factor H (CFH)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

Western Blot; Sample: Lane1: Mouse Serum; Lane2: Mouse Plasma; Lane3: Mouse Liver lysate Primary Ab: 1µg/ml Rabbit Anti-Mouse CFH Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CFH. It has been selected for its ability to recognize CFH in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Citations

Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
Circulating matrix metalloproteinases and procollagen propeptides in inguinal herniaPubmed:29484522
Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor HPubmed: 31187271
Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H33788378
A Family Affair: Addressing the Challenges of Factor H and the Related Proteins33868311