Polyclonal Antibody to Complement Factor I (CFI) Homo sapiens (Human) Polyclonal antibody

CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB978Hu02-Recombinant Complement Factor I (CFI)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Complement Factor I (CFI) Packages (Simulation)
  • Polyclonal Antibody to Complement Factor I (CFI) Packages (Simulation)
  • Polyclonal Antibody to Complement Factor I (CFI) DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CFI Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Factor I (CFI) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human CFI Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Complement Factor I (CFI) DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human CFI Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB978Hu02.jpg Figure. Western Blot; Sample: Recombinant CFI, Human.
  • Polyclonal Antibody to Complement Factor I (CFI) Figure. Western Blot; Sample: Porcine Kidney Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CFI. It has been selected for its ability to recognize CFI in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • A case report of thrombotic microangiopathy without renal involvement carrying two novel mutations in complement-regulator genesPubMed: 26613809
  • Atypical hemolytic uremic syndrome: Korean pediatric seriesPubMed: 25443527
  • NOVEL ASSAYy2016:139118
  • Compliment factor i bioassayUS20170285024
  • A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and …Pubmed:29782502
  • The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H33788378

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