Polyclonal Antibody to Corticotropin Releasing Factor (CRF) Homo sapiens (Human) Polyclonal antibody

CRH; Corticotropin Releasing Hormone; Corticoliberin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA835Hu04-Recombinant Corticotropin Releasing Factor (CRF)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Corticotropin Releasing Factor (CRF) Packages (Simulation)
  • Polyclonal Antibody to Corticotropin Releasing Factor (CRF) Packages (Simulation)
  • PAA835Hu04.jpg Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Rat Placenta lysate; Lane4: Mouse Placenta lysate Primary Ab: 0.6µg/ml Rabbit Anti-Human CRF Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CRF. It has been selected for its ability to recognize CRF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male ratsPubmed:24440345
  • Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?Pubmed:24862833
  • Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary levelPubmed:25236411
  • Acute Stress-Induced Changes in Follicular Dermal Papilla Cells and Mobilization of Mast Cells:Implications for Hair Growth.pubmed:27746640
  • Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulationpubmed:28663093
  • Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipationpubmed:29082242
  • Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female ratsPubmed:30028991
  • Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary …Pubmed:30038568
  • Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptorsPubmed:29807116
  • The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …Pubmed:29379286
  • Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine …Pubmed:29540178
  • Bioscience Research
  • Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in micePubmed: 31431066
  • Altered glucocorticoid metabolism represents a feature of macroph‐agingPubmed: 32463582
  • Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress33408312
  • O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993

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