Polyclonal Antibody to Creatine Kinase MB Isoenzyme (CKMB) Mus musculus (Mouse) Polyclonal antibody

CK2; CK-MB; Creatine Kinase,Muscle/Brain

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Creatine Kinase MB Isoenzyme (CKMB) Packages (Simulation)
  • Polyclonal Antibody to Creatine Kinase MB Isoenzyme (CKMB) Packages (Simulation)
  • Polyclonal Antibody to Creatine Kinase MB Isoenzyme (CKMB) DAB staining on IHC-P;
    Sample: Mouse Cardiac Muscle Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse CKMB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Creatine Kinase MB Isoenzyme (CKMB) Western Blot; Sample: Mouse Cerebrum lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Mouse CKMB Antibody
    Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CKMB. It has been selected for its ability to recognize CKMB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.1-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia–Reperfusion InjuryPubMed: 22814936
  • N-Acetylcysteine and Allopurinol Confer Synergy in Attenuating Myocardial Ischemia Injury via Restoring HIF-1α/HO-1 Signaling in Diabetic RatsPubMed: PMC3715528
  • Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbitsPubMed: 26629064
  • Diabetes blocks the cardioprotective effects of sevoflurane postconditioning by impairing Nrf2/Brg1/HO-1 signalingPubmed:26973173
  • Effect of levocarnitine on TIMP-1, ICAM-1 expression of rats with coronary heart disease and its myocardial protection effectPubmed:26972400
  • Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fractionpubmed:27924211
  • Peroxisome proliferator-activated receptor γ (PPARγ) mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via …PMC5209473
  • In Vivo Cardioprotective Effects and Pharmacokinetic Profile of N-Propyl Caffeamide AgainstIschemia Reperfusion Injury.pubmed:27480649
  • Cardiac biomarkers in dogs with visceral leishmaniasisS0301-732X2016000300004
  • Remote Limb Ischaemic Postconditioning Protects Against Myocardial Ischaemia/ Reperfusion Injury in Mice: Activation of JAK/STAT3-Mediated Nrf2-Antioxidant Signalling10.1159:481755
  • Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in RatsDOI: 10.5152/etd.2017.1732
  • Metformin attenuated endotoxin-induced acute myocarditis via activating AMPKpubmed:28410530
  • Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity andPubmed:29727991
  • GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic micePubmed:29605032
  • Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
  • Cyclic helix B peptide ameliorates acute myocardial infarction in mice by inhibiting apoptosis and inflammatory responses
  • Adiponectin Facilitates Postconditioning Cardioprotection through Both AMPK-Dependent Nuclear and AMPK-Independent Mitochondrial STAT3 ActivationPubmed: 32190173
  • Tissue suction-mediated gene transfer to the beating heart in micePubmed: 32027678
  • Deletion of Rap1 protects against myocardial ischemia/reperfusion injury through suppressing cell apoptosis via activation of STAT3 signalingPubmed: 32020680
  • Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injuryPubmed: 32705277
  • Hydrogen sulphide and nitric oxide cooperate in cardioprotection against ischemia/reperfusion injury in isolated rat heartPubmed: 32871779
  • Melatonin Attenuates Anoxia/Reoxygenation Injury by Inhibiting Excessive Mitophagy Through the MT2/SIRT3/FoxO3a Signaling Pathway in H9c2 CellsPubmed: 32546969
  • Biological Safety and Biodistribution of Chitosan NanoparticlesPubmed: 32340313
  • The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin?induced cardiotoxicity33300057
  • Inactivation of TOPK Caused by Hyperglycemia Blocks Diabetic Heart Sensitivity to Sevoflurane Postconditioning by Impairing the PTEN/PI3K/Akt Signaling33986917
  • Long non-coding RNA MALAT1 modulates myocardial ischemia-reperfusion injury though the PI3K/Akt/eNOS pathway by sponging miRNA-133a-3p to target IGF1R …Pubmed:34968461
  • Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic …Pubmed:35715527
  • Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733Pubmed:35215383
  • Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrestPubmed:35495584
  • MicroRNA-503 Exacerbates Myocardial Ischemia/Reperfusion Injury via Inhibiting PI3K/Akt-and STAT3-Dependent Prosurvival Signaling PathwaysPubmed:35620576
  • Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress

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