Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) Rattus norvegicus (Rat) Polyclonal antibody

MTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPD284Ra01-Recombinant Cytochrome C Oxidase Subunit II (COX2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) Packages (Simulation)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) Packages (Simulation)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P; Sample: Rat Cardiac Muscle Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Cerebrum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Pancreas Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Ovary Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Spleen Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Colon Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Ovary Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Pancreas Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytochrome C Oxidase Subunit II (COX2) DAB staining on IHC-P;
    Sample: Rat Spleen Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat COX2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAD284Ra01.jpg Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Liver lysate; Lane3: Rat Cerebrum lysate Primary Ab: 1μg/ml Rabbit Anti-Rat COX2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against COX2. It has been selected for its ability to recognize COX2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Molecular Mechanisms Underlying Chemopreventive Activities of Glycyrrhizic Acid against UVB- Radiation-Induced Carcinogenesis in SKH-1 Hairless Mouse EpidermisPubMed: 21545294
  • Diallyl sulfide protects against ultraviolet B-induced skin cancers in SKH-1 hairless mouse: analysis of early molecular events in carcinogenesisWiley: source
  • Discovery of a novel anti-cancer agent targeting both topoisomerase I and II in hepatocellular carcinoma Hep3B cells in vitro and in vivo: Cinnamomum verum component 2-methoxycinnamaldehyde.Pubmed:26707867
  • Discovery of a novel anticancer agent with both anti-topoisomerase I and II activities in hepatocellular carcinoma SK-Hep-1 cells in vitro and in vivo: Cinnamomum verum component 2-methoxycinnamaldehydePubmed:26792981
  • The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female.pubmed:28174048
  • Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus.pubmed:28553224
  • Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effectspubmed:29102164
  • Effect of single and repeated heat stress on chemical signals of heat shock response cascade in the rat's heart.pubmed:29178005
  • Quercetin‐3‐methyl ether inhibits esophageal carcinogenesis by targeting the AKT/mTOR/p70S6K and MAPK pathwaysPubmed:30035335
  • Celecoxib prevents cognitive impairment and neuroinflammation in soluble Amyloid β-treated ratsPubmed:29306052
  • Polar extract of protects cartilage homeostasis: possible mechanism of actionPubmed:29313174
  • Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and …Pubmed:29678767
  • Barrier Strengthening and Anti-inflammatory Effect of Cucurbit Fruits on Intestinal Epithelial Cells In-vitro10.2174:1573401313666170427123153
  • Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanismsPubmed: 30456649
  • Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanismsPubmed: 30421246
  • Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard &
  • 1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathwayPubmed: 30928409
  • Polydatin ameliorates chemotherapy-induced cognitive impairment (chemobrain) by inhibiting oxidative stress, inflammatory response, and apoptosis in ratsPubmed: 31992173
  • Dietary Supplementation of Foxtail Millet Ameliorates Colitis-Associated Colorectal Cancer in Mice via Activation of Gut Receptors and Suppression of the STAT3 …Pubmed: 32784751
  • Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes MellitusPubmed: 33123597
  • Rewiring of chemically modified MAPK pathway and its downstream targets by rosmarinic acid in mice dermal cancer
  • Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung
  • Targeting NF-¦ÊB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro33598414
  • Neuroprotective Effect of Clobenpropit against Lipopolysaccharide-Induced Cognitive Deficits via Attenuating Neuroinflammation and Enhancing Mitochondrial …34942919

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