Polyclonal Antibody to Cytochrome b-245 Beta Polypeptide (CYBb) Homo sapiens (Human) Polyclonal antibody

CGD; GP91PHOX; NOX2; Neutrophil cytochrome b91; Cytochrome b558 beta; Heme-binding membrane glycoprotein gp91phox; Superoxide-generating NADPH oxidase heavy chain

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPD308Hu01-Recombinant Cytochrome b-245 Beta Polypeptide (CYBb)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.32mg/mL
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  • Polyclonal Antibody to Cytochrome b-245 Beta Polypeptide (CYBb) Packages (Simulation)
  • Polyclonal Antibody to Cytochrome b-245 Beta Polypeptide (CYBb) Packages (Simulation)
  • Polyclonal Antibody to Cytochrome b-245 Beta Polypeptide (CYBb) IHC Images
  • PAD308Hu01.jpg Figure. Western Blot; Sample: Recombinant CYBb, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CYBb. It has been selected for its ability to recognize CYBb in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Low dose of acetylsalicylic acid and oxidative stress-mediated endothelial dysfunction in diabetes: a short-term evaluationPubmed:25091345
  • Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndromepubmed:27538909
  • NOX5-induced uncoupling of endothelial NO synthase is a causal mechanism and theragnostic target of an age-related hypertension endotypePubmed: 33170835

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