Polyclonal Antibody to Epidermal Growth Factor Receptor (EGFR)    
    
ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Overview
Properties
- Product No.PAA757Hu02
 - Organism SpeciesHomo sapiens (Human) Same name, Different species.
 - ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
 - CategoryCD & Adhesion moleculeTumor immunity
 
- SourcePolyclonal antibody preparation, Host Rabbit
 - Ig Type IgG, Potency n/a
 - PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
 - LabelNone
 - Immunogen RPA757Hu02-Recombinant Epidermal Growth Factor Receptor (EGFR)
 - Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
 - TraitsLiquid, Concentration 0.5mg/mL
 
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Specifity
The antibody is a rabbit polyclonal antibody raised against EGFR. It has been selected for its ability to recognize EGFR in immunohistochemical staining and western blotting.
Usage
            Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Sus scrofa; Porcine (Pig)Giveaways
Increment services
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                    Antibody Labeling Customized Service
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                    Protein A/G Purification Column
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                    Staining Solution for Cells and Tissue
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                    Positive Control  for Antibody
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                    Tissue/Sections Customized Service
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                    Phosphorylated Antibody Customized Service
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                    Western Blot (WB) Experiment Service
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                    Immunohistochemistry (IHC) Experiment Service
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                    Immunocytochemistry (ICC) Experiment Service
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                    Flow Cytometry (FCM) Experiment Service
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                    Immunoprecipitation (IP) Experiment Service
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                    Immunofluorescence (IF) Experiment Service
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                    Buffer
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                    DAB Chromogen Kit
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                    SABC Kit
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                    Long-arm Biotin Labeling Kit
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                    Real Time PCR Experimental Service
                                 
Citations
- Nimotuzumab combined with gemcitabine and cisplatin as second-line chemotherapy for advanced non-small-cell lung cancerWiley: source
 - Increased Epidermal Growth Factor Receptor (EGFR) Associated with Hepatocyte Growth Factor (HGF) and Symptom Severity in Children with Autism …Pubmed:25249767
 - GC1118, an anti-EGFR antibody with a distinct binding epitope and superior inhibitory activity against high-affinity EGFR ligandsPubMed: 26586721
 - LINC01225 promotes occurrence and metastasis of hepatocellular carcinoma in an epidermal growth factor receptor-dependent pathwayPubmed:26938303
 - Microfluidic immunosensor based on mesoporous silica platform and CMK-3/poly-acrylamide-co-methacrylate of dihydrolipoic acid modified gold electrode for cancer biomarker detection.pubmed:28335979
 - EGFR detection in extracellular vesicles of breast cancer patients through immunosensor based on silica-chitosan nanoplatformDoi: 10.1016/j.talanta.2018.10.016
 - Design, synthesis, molecular docking and biological activity evaluation of some novel indole derivatives as potent anticancer active agents and apoptosis inducersPubmed: 30665034
 - Novel thienopyrimidine derivatives as dual EGFR and VEGFR-2 inhibitors: design, synthesis, anticancer activity and effect on cell cycle profilePubmed: 30919701
 - Design and synthesis of new benzoxazole/benzothiazole-phthalimide hybrids as antitumor-apoptotic agents
 - Synthesis and anticancer activity of bis-benzo [d][1, 3] dioxol-5-yl thiourea derivatives with molecular docking studyPubmed: 31288134
 - Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters …Pubmed: 31737175
 - Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicinePubmed: 33212364
 - Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung
 - Synthesis, EGFR-TK inhibition and anticancer activity of new quinoxaline derivatives
 - Humoral immune response to epidermal growth factor receptor in lung cancer33495907
 - Pyrazolo[3,4-d]pyrimidine-based dual EGFR T790M/HER2 inhibitors: Design, synthesis, structure¨Cactivity relationship and biological activity as potential antitumor and anticonvulsant agents33545637
 - Design, synthesis, antiproliferative evaluation, and molecular docking study of new quinoxaline derivatives as apoptotic inducers and EGFR inhibitors
 - Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions34914377
 - Discovery of new pyrimido [5, 4-c] quinolines as potential antiproliferative agents with multitarget actions: Rapid synthesis, docking, and ADME studiesPubmed:35219045
 
