Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) Mus musculus (Mouse) Polyclonal antibody

EMMPRIN; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB540Mu01-Recombinant Cluster Of Differentiation 147 (CD147)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) Packages (Simulation)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) Packages (Simulation)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) DAB staining on IHC-P;
    Samples: Mouse Testis Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) DAB staining on IHC-P;
    Samples: Mouse Kidney Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) DAB staining on IHC-P;
    Samples: Mouse Heart Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) DAB staining on IHC-P;
    Samples: Mouse Uterus Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) DAB staining on IHC-P;
    Samples: Mouse Intestine Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB540Mu01.jpg Figure. Western Blot; Sample: Recombinant EMMPRIN, Mouse.
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse CD147 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) Western Blot; Sample: Rat Heart lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) Mouse Thymocytes cell were stained with 20µg/ml rabbit Anti-mouse CD147 Polyclonal Antibody (Catalog PAB540Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CD147. It has been selected for its ability to recognize CD147 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • The Combination of CD147 and MMP-9 Serum Levels Is Identified as Novel Chemotherapy Response Markers of Advanced Non-Small-Cell Lung CancerPubmed: 32377273

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