Polyclonal Antibody to Filaggrin (FLG) Mus musculus (Mouse) Polyclonal antibody

Profilaggrin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPJ103Mu02-Recombinant Filaggrin (FLG)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Filaggrin (FLG) Packages (Simulation)
  • Polyclonal Antibody to Filaggrin (FLG) Packages (Simulation)
  • Polyclonal Antibody to Filaggrin (FLG) Western Blot; Sample: Mouse Skin lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse FLG Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against FLG. It has been selected for its ability to recognize FLG in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Filaggrin R501x and 2282del4 gene mutations and its tissue levels in patients with chronic plaque psoriasisLWW: 01000
  • Neuromodulatory and Anti-Inflammatory Ingredient for Sensitive Skin: In Vitro Assessment Pubmed:24931812
  • Protective Effects of Triphala on Dermal Fibroblasts and Human KeratinocytesPubmed:26731545
  • In vitro anti-inflammatory and skin protective properties of extract comprising ceramides from de-oiled rice brandoi:10.1111
  • Imiquimod-induced psoriasis-like inflammation in differentiated Human keratinocytes: Its evaluation using curcumin.pubmed:28736282
  • A novel, topical, nonsteroidal, TRPV1 antagonist, PAC-14028 cream improves skin barrier function and exerts anti-inflammatory action through modulating epidermal …Pubmed:29752146
  • In vitro anti-inflammatory and skin protective properties of Virgin coconut oil10.1016:j.jtcme.2017.06.012
  • Markers of atopic dermatitis, allergic rhinitis and bronchial asthma in pediatric patients: correlation with filaggrin, eosinophil major basic protein and …
  • Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitisPubmed: 30560019
  • Epidermal and Dermal Hallmarks of Photoaging are Prevented by Treatment with Night Serum Containing Melatonin, Bakuchiol, and Ascorbyl …
  • 糖化に起因する表皮機能低下に対するワイルドタイムエキスの防御効果
  • Filaggrin‐gene mutation has minimal effect on the disease severity in the lesions of atopic dermatitis34322929

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