Polyclonal Antibody to Forkhead Box P3 (FOXP3) Rattus norvegicus (Rat) Polyclonal antibody

FOX-P3; AIID; DIETER; IPEX; JM2; PIDX; XPID; SCURFIN; Forkhead Box Protein P3; Immune Dysregulation,Polyendocrinopathy,Enteropathy,X-Linked

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB877Ra01-Recombinant Forkhead Box P3 (FOXP3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.63mg/ml
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  • Polyclonal Antibody to Forkhead Box P3 (FOXP3) Packages (Simulation)
  • Polyclonal Antibody to Forkhead Box P3 (FOXP3) Packages (Simulation)
  • Polyclonal Antibody to Forkhead Box P3 (FOXP3) Western Blot; Sample: Rat Cerebrum lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Rat FOXP3 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against FOXP3. It has been selected for its ability to recognize FOXP3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Dichloroacetate at therapeutic concentration alters glucose metabolism and induces regulatory T-cell differentiation in alloreactive human lymphocytesPubmed: 23612652
  • Genetic Polymorphisms of Foxp3 in Patients with Rheumatoid ArthritisPubmed:25448791#
  • Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patientsarticle:30785
  • The Role of T-Regulatory Expression in Autoimmune Thyroid Disease and its Association with Thyroid _x005f_x0004_Antibodythe-role-of-tregulatory-expression-in-autoimmune-t
  • gene polymorphisms and its protein level in patients with rheumatoid arthritis: a case–control studyPubmed:29411043
  • Therapeutic potential of tranilast for the treatment of chronic graft-versus-host disease in micePubmed: 30307955
  • Toll-Like Receptor 4–Myeloid Differentiation Primary Response Gene 88 Pathway Is Involved in the Shikonin Treatment of CIA by Regulating Treg/Th17 ExpressionDoi: 10.1155/2018/2428546
  • Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration ¡­33577585

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