Polyclonal Antibody to G Protein Coupled Estrogen Receptor 1 (GPER) Mus musculus (Mouse) Polyclonal antibody

GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPG045Mu01-Recombinant G Protein Coupled Estrogen Receptor 1 (GPER)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to G Protein Coupled Estrogen Receptor 1 (GPER) Packages (Simulation)
  • Polyclonal Antibody to G Protein Coupled Estrogen Receptor 1 (GPER) Packages (Simulation)
  • Polyclonal Antibody to G Protein Coupled Estrogen Receptor 1 (GPER) AF488 staining on IF;
    Sample: NIH/3T3 cell
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPER Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • Polyclonal Antibody to G Protein Coupled Estrogen Receptor 1 (GPER) Western Blot; Samples: Lane1: A549 cell lysate; Lane2: Hela cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Mouse GPER Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GPER. It has been selected for its ability to recognize GPER in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signallingPubmed:24846829
  • Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cellsPubmed:24859647
  • Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signallingPubMed: 24846831
  • Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblastsscience:S1001074216300924
  • Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder.pubmed:27512921
  • Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Naïve Patients with Major Depressive Disorderpubmed:29073745
  • Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders.pubmed:28734238
  • serum levels of gPer-1 in euthymic bipolar patientsPubmed:29618927
  • An evaluation of G-protein coupled membrane estrogen receptor-1 level in stutteringPubmed:29299745
  • Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-naïve patients with attention deficit hyperactivity disorder (ADHD)Pubmed:29659348
  • In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts
  • GPER-1 and sex-hormone levels in patients with otosclerosisPubmed: 32144019
  • Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertilityPubmed: 32776559
  • GPER-1 in chronic hepatitis B
  • Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota?¡­34085740
  • Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study
  • The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus34787384

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