Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) Rattus norvegicus (Rat) Polyclonal antibody

AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA725Ra01-Recombinant Glucose 6 Phosphate Isomerase (GPI)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) Packages (Simulation)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) Packages (Simulation)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) DAB staining on IHC-P; Samples: Rat Cerebrum Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat GPI Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) DAB staining on IHC-P;
    Samples: Rat Stomach Tissue
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) DAB staining on IHC-P;
    Samples: Rat Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat GPI Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) DAB staining on IHC-P;
    Samples: Rat Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat GPI Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) DAB staining on IHC-P;
    Samples: Rat Spleen Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Rat GPI Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA725Ra01.jpg WB Image
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) Western Blot; Sample: Human PC3 cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Rat GPI Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) Western Blot; Sample: Rat Serum;
    Primary Ab: 3µg/ml Rabbit Anti-Rat GPI Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) Western Blot; Sample: Rat Prostate lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Rat GPI Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Rat GPI Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GPI. It has been selected for its ability to recognize GPI in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factorPubMed: 23027359
  • Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex‐mediated inflammatory arthritisPubmed:26945549
  • Bruton's Tyrosine Kinase Deficiency Inhibits Autoimmune Arthritis in Mice but Fails to Block Immune Complex–Mediated Inflammatory Arthritispubmed:26945549
  • The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malariaPubmed:29784863
  • Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motilityPubmed: 31394311
  • The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI …Pubmed: 31959860
  • T–B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated ProteinPubmed: 33199538

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