Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Homo sapiens (Human) Polyclonal antibody

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA822Hu02-Recombinant Heat Shock Protein 60 (Hsp60)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Human Stomach cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA822Hu02.jpg Figure. Western Blot; Sample: Recombinant HSPD1, Human.
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Figure. Western Blot; Sample: Rat Liver Tissue.
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HSPD1 Polyclonal Antibody (Catalog # PAA822Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Human Hela cell was stained with 20µg/ml rabbit Anti-human Hsp60 Polyclonal Antibody (Catalog PAA822Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against Hsp60. It has been selected for its ability to recognize Hsp60 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Hsp60 response in experimental and human temporal lobe epilepsyPubMed: 25801186
  • Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitroPubmed: 30878663
  • Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970

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