Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Mus musculus (Mouse) Polyclonal antibody

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA822Mu02-Recombinant Heat Shock Protein 60 (Hsp60)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Mouse Ovary Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Figure. DAB staining on IHC-P;
    Samples: Mouse Spleen Tissue.
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Mouse Uterus Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Sample: Mouse Uterus Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse Hsp60 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse Hsp60 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Figure: FITC staining on IHC-P;
    Sample: Hela cells.
  • PAA822Mu02.jpg Figure. Western Blot; Sample: Recombinant HSPD1, Mouse.
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Mouse Lung lysate; Lane3: Hela cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Mouse Hsp60 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against Hsp60. It has been selected for its ability to recognize Hsp60 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Hsp60 response in experimental and human temporal lobe epilepsyPubMed: 25801186
  • Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitroPubmed: 30878663
  • Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970

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