Polyclonal Antibody to High Molecular Weight Kininogen (HMWK) 
Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor
Overview
Properties
- Product No.PAB805Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseHematology
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB805Ra01-Recombinant High Molecular Weight Kininogen (HMWK)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 0.5mg/ml
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat HMWK Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Ovary Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat HMWK Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant HMWK, Rat.
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Western Blot; Sample: Rat Kidney lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat HMWK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Heart lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat HMWK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat HMWK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Placenta lysate;
Primary Ab: 1µg/ml Rabbit Anti-Rat HMWK Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Serum
Primary Ab: 0.1µg/ml Rabbit Anti-Rat KNG1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against HMWK. It has been selected for its ability to recognize HMWK in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
Citations
- Factor XII–mediated contact activation related to poor prognosis in disseminated intravascular coagulationPubMed: 26706311
- Factor XII-mediated contact activation related to poor prognosis in disseminated intravascular coaCavia (Guinea pig )lationPubmed:26706311
- Contact system activation and high thrombin generation in hyperthyroidism.pubmed:28137735
- Contact system activation in disseminated intravascular coagulation: activities of prekallikrein and high-molecular-weight kininogen are significant risk factorsPubmed:34993714