Recombinant High Molecular Weight Kininogen (HMWK) Rattus norvegicus (Rat) Recombinant protein

Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Molecular Mass 61.1kDa, Accurate 59kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu214~Asp475 with N-terminal His and GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point6.5
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  • Recombinant High Molecular Weight Kininogen (HMWK) Packages (Simulation)
  • Recombinant High Molecular Weight Kininogen (HMWK) Packages (Simulation)
  • RPB805Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Factor XII–mediated contact activation related to poor prognosis in disseminated intravascular coagulationPubMed: 26706311
  • Factor XII-mediated contact activation related to poor prognosis in disseminated intravascular coaCavia (Guinea pig )lationPubmed:26706311
  • Contact system activation and high thrombin generation in hyperthyroidism.pubmed:28137735
  • Contact system activation in disseminated intravascular coagulation: activities of prekallikrein and high-molecular-weight kininogen are significant risk factorsPubmed:34993714

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