Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) Homo sapiens (Human) Polyclonal antibody

HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA798Hu02-Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.32mg/mL
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  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) Packages (Simulation)
  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) Packages (Simulation)
  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human HIF1a Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HIF1a Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HIF1a Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human HIF1a Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA798Hu02.jpg Figure. Western Blot; Sample: Recombinant HIF1a, Human.
  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) Figure. Western Blot; Sample: Human Serum.
  • Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) Western Blot; Sample: MCF7 cell lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Human HIF1a Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HIF1a. It has been selected for its ability to recognize HIF1a in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells.PubMed: 19466611
  • Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibitionScienceDirect: S0014299909011352
  • Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor PrognosisPubMed: PMC3781153
  • Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic ratsPubmed: 24002353
  • Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemiaPubmed: 24289078
  • Method for preventing or treating a disease, disorder or condition induced by retina ischemiaUS8569372
  • Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats.Pubmed: 23816242
  • HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa KeratitisPlos: Source
  • Telomeres are elongated in rats exposed to moderate altitudePubmed:Pmc4088304
  • Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein.Pubmed:25384583
  • The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGFPubmed:25445537
  • Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic ratsPubmed:25524396
  • Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birthPubMed: 25889224
  • Determination of the Preferred Conditions for the Isolated Perfusion of Porcine KidneysPubMed: 25341590
  • Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic systemPubMed: 25905434
  • Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariaepmc:PMC4794592
  • Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1αPubmed:27121973
  • Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией91
  • Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α.pubmed:27121973
  • Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritisetm:13
  • Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trialissn:2542-3975
  • R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme pubmed:28667042
  • Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia?10.1007/s00404-017-4461-2
  • Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in kneepubmed:28123469
  • Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation10.1016/j.mjafi.2017.04.003
  • Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxiaPubmed:29454386
  • Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate HypoxiaPubmed:29308565
  • Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticlesPubmed: 30209641
  • Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
  • OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor GrowthPubmed: 30468988
  • Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.
  • Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar RatsPubmed: 30903510
  • The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency
  • Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathwayPubmed: 31055848
  • A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A …Pubmed: 31020669
  • Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder AugmentationPubmed: 31238789
  • ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ
  • Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsiaPubmed: 31420792
  • Regulation of MicroRNA-155 and Its Related Genes Expression by Inositol Hexaphosphate in Colon Cancer CellsPubmed: 31744065
  • Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callusPubmed: 32894200
  • PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancerPubmed: 32383180
  • Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза
  • Prenatal vitamin D supplementation reduces blood pressure and improves placental angiogenesis in an animal model of preeclampsia33237074
  • Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ¡­33705802
  • Astaxanthin mediated regulation of VEGF through HIF1¦Á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model33789142
  • Serum hypoxia-inducible factor-1¦Á and uterine artery Doppler ultrasound during the first trimester for prediction of preeclampsia33758274
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377

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