Polyclonal Antibody to Insulin Receptor Substrate 1 (IRS1)

HIRS1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 108.00 US$ 252.00 US$ 360.00 US$ 900.00 US$ 3,600.00
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Overview

Properties

Product No.PAC546Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEndocrinology

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC546Mu01-Recombinant Insulin Receptor Substrate 1 (IRS1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Sample: Human Hela cell; Primary Ab: 20μg/ml Rabbit Anti-Mouse IRS1 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)

Western Blot; Sample: Rat Cerebrum lysate Primary Ab: 2μg/ml Rabbit Anti-Mouse IRS1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Liver lysate
Primary Ab: 1µg/ml Rabbit Anti-Mouse IRS1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Bovine Cerebrum lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse IRS1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IRS1. It has been selected for its ability to recognize IRS1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Bos taurus; Bovine (Cattle)

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Citations

Сравнительный анализ уровней антител к инсулиновым рецепторам у детей с сахарным диабетом i типа в зависимости от стадии заболеванияarticle:n
Regulation of insulin receptor phosphorylation in the brains of prenatally stressed rats: New insight into the benefits of antidepressant drug treatment.pubmed:28063625
Lack of brain insulin receptor substrate-1 causes growth retardation, with decreased expression of growth hormone-releasing hormone in the hypothalamus.33980693