Polyclonal Antibody to Interferon Regulatory Factor 4 (IRF4)

LSIRF; MUM1; NF-EM5; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 103.00 US$ 239.00 US$ 342.00 US$ 855.00 US$ 3,420.00
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Overview

Properties

Product No.PAB755Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Infection immunity Immune molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB755Mu01-Recombinant Interferon Regulatory Factor 4 (IRF4)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

Used in DAB staining on fromalin fixed paraffin-embedded Spleen tissue

Figure. Western Blot; Sample: Recombinant IRF4, Mouse.
Western Blot; Sample: Lane1: Mouse Spleen lysate; Lane2: Mouse Thymus lysate; Lane3: Mouse Lymph node lysate; Lane4: Rat Spleen lysate; Lane5: RAW264.7 cell lysate
Primary Ab: 0.6µg/ml Rabbit Anti-Mouse IRF4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Spleen lysate; Lane2: Mouse Lymph node lysate; Lane3: Rat Spleen lysate; Lane4: RAW264.7 cell lysate; Lane5: U937 cell lysate； Lane6: K562 cell lysate
Primary Ab: 0.6µg/ml Rabbit Anti-Mouse IRF4 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IRF4. It has been selected for its ability to recognize IRF4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.