Polyclonal Antibody to Interleukin 33 (IL33) Rattus norvegicus (Rat) Polyclonal antibody

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB980Ra02-Recombinant Interleukin 33 (IL33)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Interleukin 33 (IL33) Packages (Simulation)
  • Polyclonal Antibody to Interleukin 33 (IL33) Packages (Simulation)
  • Polyclonal Antibody to Interleukin 33 (IL33) IHC Images
  • PAB980Ra02.jpg Figure. Western Blot; Sample: Recombinant IL33, Rat.
  • Polyclonal Antibody to Interleukin 33 (IL33) Western Blot; Sample: Lane1: Rat Small intestine lysate; Lane2: Rat Uterus lysate; Lane3: Rat Kidney lysate
    Primary Ab: 2µg/mL Rabbit Anti-Rat IL33 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interleukin 33 (IL33) Western Blot; Samples: Lane1: Rat Lung cells treated with 1µg/ml LPS for 24 hours; Lane2: Untreated Rat Lung lysate;
    Primary Ab: 0.2µg/ml Rabbit Anti-Rat IL33 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interleukin 33 (IL33) Western Blot; Sample: Lane1: Rat Kidney lysate; Lane2: Rat Cerebrum lysate
    Primary Ab: 2μg/ml Rabbit Anti-Rat IL33 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IL33. It has been selected for its ability to recognize IL33 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Interleukin-33, matrix metalloproteinase-9, and tissue ınhibitor of matrix metalloproteinase-1 in myocardial infarctionPubMed: PMC3604606
  • CXCL13 blockade attenuates lupus nephritis of MRL/lpr micePubMed: 26456520
  • Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic RhinosinusitisPubmed:26540312
  • The Expression and Regulation of Interleukin-33 in Human Epidermal Keratinocytes: A New Mediator of Atopic Dermatitis and Its Possible Signaling Pathway.pubmed:27348082
  • IL-33 circulating serum levels are increased in patients with non-segmental generalized vitiligopubmed:27388717
  • Chrysin Protects Rat Kidney from Paracetamol-Induced Oxidative Stress, Inflammation, Apoptosis, and Autophagy: A Multi-Biomarker Approach. pubmed:28134775
  • Association of IL-33, IL1RL1 gene polymorphisms with serum IL-33 levels and risk of asthma in adults and asthmatic bronchitis in children (Chinese)10.1080:13102818.2018.1471361
  • Role of IL-33 and ST2 signaling and inflammatory responses in non-small cell lung cancer172341
  • Group II innate lymphoid cells and microvascular dysfunction from pulmonary titanium dioxide nanoparticle exposurePubmed: 30413212
  • Interleukin-33 serum levels in postmenopausal women with osteoporosisPubmed: 30846811
  • Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2sPubmed: 32101749
  • Relationship of microbial profile with airway immune response in eosinophilic or neutrophilic inflammation of asthmaticsPubmed: 32141256
  • Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
  • Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
  • Inhibition of NF-κB/IL-33/ST2 Axis Ameliorates Acute Bronchiolitis Induced by Respiratory Syncytial Virus34395633
  • Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus PatientsPubmed:35056005
  • Dexmedetomidine Attenuates Hyperalgesia Induced By Brachial Plexus Root Avulsion By Restoring The GLT-1 Function Via PKA Signaling

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