• KO-Validated
Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) Homo sapiens (Human) Polyclonal antibody

F5F8D; FMFD1; MCFD1; MR60; Gp58; Protein ERGIC-53; Coagulation Factor V-Factor VIII Combined Deficiency; Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC567Hu01-Recombinant Lectin, Mannose Binding 1 (LMAN1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) Packages (Simulation)
  • Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) Packages (Simulation)
  • Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human LMAN1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) FITC staining on IF;
    Sample: Human Hela cell;
    Primary Ab: 20µg/ml Rabbit Anti-Human LMAN1 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAC567Hu01.jpg Western Blot; Sample: Human HepG2 cell lysate; Primary Ab: 1µg/ml Rabbit Anti-Human LMAN1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) Western Blot; Samples: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: MCF7 cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human LMAN1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: LMAN1 knockout Hela cell lysate;
    Predicted MW: 58kd
    Observed MW: 58kd
    Primary Ab: 1µg/ml Rabbit Anti-Human LMAN1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Lectin, Mannose Binding 1 (LMAN1) Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human LMAN1 Polyclonal Antibody (Catalog PAC567Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against LMAN1. It has been selected for its ability to recognize LMAN1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence: 5-20µg/mL;
Flow cytometry: 20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

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