KO-Validated
Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 2 (MAP2K2)

MAPKK2; MEK2; MKK2; PRKMK2; ERK activator kinase 2; Dual specificity mitogen-activated protein kinase kinase 2

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
Quantity

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Overview

Properties

Product No.PAD562Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD562Hu01-Recombinant Mitogen Activated Protein Kinase Kinase 2 (MAP2K2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Samples: Human Hela cell; Primary Ab: 20μg/ml Rabbit Anti-Human MAP2K2 Antibody Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)

Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: A549 cell lysate Primary Ab: 0.3µg/ml Rabbit Anti-Human MAP2K2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: MCF7 cell lysate; Lane3: Jurkat cell lysate; Lane4: Hela cell lysate; Lane5: Porcine Cerebrum lysate
Primary Ab: 2µg/ml Rabbit Anti-Human MAP2K2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: MAP2K2 knockout Hela cell lysate;
Predicted MW: 44kd
Observed MW: 44kd
Primary Ab: 5µg/ml Rabbit Anti-Human MAP2K2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Bovine Lung lysate; Lane3: Gallus Lung lysate; Lane4: Equine Lung lysate
Primary Ab: 0.5µg/ml Rabbit Anti-Human MAP2K2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MAP2K2. It has been selected for its ability to recognize MAP2K2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus)