Polyclonal Antibody to SMAD family member 5 (SMAD5) Homo sapiens (Human) Polyclonal antibody

MADH5; Dwfc; JV5-1; Mothers Against Decapentaplegic Homolog 5

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC122Hu01-Recombinant SMAD family member 5 (SMAD5)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to SMAD family member 5 (SMAD5) Packages (Simulation)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) Packages (Simulation)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) DAB staining on IHC-P;
    Samples: Human Lung cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human Smad5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) DAB staining on IHC-P;
    Sample: Human Breast cancer Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human SMAD5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) DAB staining on IHC-P;
    Sample: Human Colorectal cancer Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human SMAD5 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC122Hu01.jpg Figure. Western Blot; Sample: Recombinant Smad5, Human.
  • Polyclonal Antibody to SMAD family member 5 (SMAD5) Western Blot; Samples: Lane1: K562 cell lysate; Lane2: Hela cell lysate; Lane3: Jurkat cell lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Human SMAD5 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SMAD5. It has been selected for its ability to recognize SMAD5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

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