• KO-Validated
Polyclonal Antibody to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Mus musculus (Mouse) Polyclonal antibody

AVP; AGTAVPRL; AII; AII/AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; MWS; NALP3; PYPAF1; Cryopyrin; Cold Induced Autoinflammatory Syndrome 1; Caterpiller-Like Receptor 1.1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPK115Mu01-Recombinant NLR Family, Pyrin Domain Containing Protein 3 (NLRP3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.37mg/mL
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  • Polyclonal Antibody to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Packages (Simulation)
  • Polyclonal Antibody to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Packages (Simulation)
  • Polyclonal Antibody to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) IHC Images
  • PAK115Mu01.jpg Figure. Western Blot; Sample: Recombinant NLRP3, Mouse.
  • Polyclonal Antibody to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Knockout Varification:
    Lane 1: Wild-type K562 cell lysate;
    Lane 2: NLRP3 knockout K562 cell lysate;
    Predicted MW: 96,112,118kDa
    Observed MW: 130kDa
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NLRP3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse NLRP3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against NLRP3. It has been selected for its ability to recognize NLRP3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Inflammatory Consequences of Maternal Diabetes on the Offspring Brain: a Hippocampal Organotypic Culture StudyPubmed: 31197747
  • Anti-Inflammatory Effect of Cherry Extract Loaded in Polymeric Nanoparticles: Relevance of Particle Internalization in Endothelial CellsPubmed: 31569594
  • Effect of combined periodontal-orthodontic treatment on NOD-like receptor protein 3 and high mobility group box-1 expressions in patients with periodontitis …Pubmed: 31689812
  • Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi

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