Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) Homo sapiens (Human) Polyclonal antibody

RAC3; ACTR; AIB1; CAGH16; CTG26; SRC3; TNRC14; TNRC16; TRAM1; pCIP; Amplified In Breast 1; Steroid Receptor Coactivator-3 ; Thyroid Hormone Receptor Activator Molecule 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPE798Hu01-Recombinant Nuclear Receptor Coactivator 3 (NCOA3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.48mg/ml
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  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) Packages (Simulation)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) Packages (Simulation)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human NCOA3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human NCOA3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human NCOA3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human NCOA3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) DAB staining on IHC-P;
    Sample: Human Breast cancer Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human NCOA3 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) DAB staining on IHC-P;
    Sample: Human Glioma Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human NCOA3 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Nuclear Receptor Coactivator 3 (NCOA3) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human NCOA3 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAE798Hu01.jpg Figure. Western Blot; Sample: Recombinant NCOA3, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against NCOA3. It has been selected for its ability to recognize NCOA3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:240-900
Immunohistochemistry: 5-20µg/mL;1:24-90
Immunocytochemistry: 5-20µg/mL;1:24-90
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer inurine samples.pubmed:27203388
  • A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample

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