Polyclonal Antibody to Osteocalcin (OC)
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Overview
Properties
- Product No.PAA471Ga01
- Organism SpeciesChicken (Gallus) Same name, Different species.
- ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayEndocrinologyHormone metabolismBone metabolism
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA471Ga01-Recombinant Osteocalcin (OC)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 0.5mg/ml
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Specifity
The antibody is a rabbit polyclonal antibody raised against OC. It has been selected for its ability to recognize OC in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Surface engineering of titanium with potassium hydroxide and its effects on the growth behavior of mesenchymal stem cellsPubMed: 19963080
- Biochemical study of the influence of ad type biophytomodulators in healing of bone defects in rats.Analelesnbc: source
- Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter?Ajas: source
- Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacityScienceDirect: S0899900709004717
- Osteogenic activity of silymarin through enhancement of alkaline phosphatase and osteocalcin in osteoblasts and tibia-fractured miceRsm: source
- Oxytocin promotes bone formation during the alveolar healing process in old acyclic female ratsScienceDirect: S0003996912001021
- Regulation of the differentiation of mesenchymal stem cells in vitro and osteogenesis in vivo by microenvironmental modification of titanium alloy surfacesScienceDirect: S0142961212000865
- Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in OsteocytesPubMed: 23357248
- Serum changes induced by intramedullar experimental administration of bisphosphonatesPubMed: 21424089
- Valvular osteoclasts in calcification and aortic valve stenosis severityPubMed: 23452891
- Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasonePubMed: 23211309
- Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in miceWiley: Source
- Puerarin promotes osteogenesis and inhibits adipogenesis in vitroPubMed: PMC3765709
- Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cellsPubMed: PMC3611891
- Morphological changes of the cartilage and bone in newborn piglets evoked by experimentally induced glucocorticoid excess during pregnancyPubmed: 22716040
- The use of Tri-Calcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical studyPubmed: 24485270
- Effect of the “protein diet” and bone tissue.Pubmed: 24483972
- Valvular osteoclasts in calcification and aortic valve stenosis severity.Pubmed: 23452891
- Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7Onlinelibrary: fsn3.109
- Effect of the “protein diet” and bone tissueAulamedica: Source
- Toward the Development and Characterization of an Easy Handling Sheet-Like Biohybrid Bone SubstitutePubmed:25761235
- Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory ConditionsWiley:Source
- Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7Pubmed:Pmc4221832
- The use of TriCalcium Phosphate (TCP) and stem cells for the regeneration of osteoperiosteal critical-size mandibular bony defects, an in vitro and preclinical studyPubmed:24485270
- Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of ratsPubmed:25038619
- Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cellsPubmed:25542352
- Pathophysiological mechanism of bone loss in type 2 diabetes involves inverse regulation of osteoblast function by PPARγ coactivator-1α and skeletal muscle atrogenes: adiponectin receptor 1 as a potential target for reversing diabetes-induced osteopeniaPubmed:25633418
- Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiationPubmed:25678785
- Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat modelsPubmed:25679008
- Inhibition of osteoclast activation by phloretin through disturbing αvβ3 integrin-c-Src pathwayPubmed:25834823
- The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in ratsPubMed: 26569440
- Biological Evaluation of a Prototype Material made of Polyglycolic Acid and HydroxyapatiteArticle: Jhtb
- Products of dentin matrix protein-1 degradation by interleukin-1β-induced matrix metalloproteinase-3 promote proliferation of odontoblastic cellsPubMed: 26355224
- Body fat loss induced by calcium in co-supplementation with conjugated linoleic acid is associated with increased expression of bone formation genes in adult micePubMed: 26454511
- Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathwayPubMed: 26677102
- Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathwayPubmed:27347050
- Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Ratsprofile:303311214
- Enhanced biocompatible and hemocompatible nano/micro porous surface as a biological scaffold for functionalizational and biointegrated implantsscience:S0045653516302016
- The effects of strength training and raloxifene on bone health in aging ovariectomized ratsPubmed:26812611
- Effect of Sodium Fluoride on Bone Biomechanical and Histomorphometric Parameters and on Insulin Signalingand Insulin Sensitivity in Ovariectomized Rats.Pubmed:26876375
- Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens journalprintahead:87
- Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickensview:87
- MiR-142-5p promotes bone repair by maintaining osteoblast activityPubmed:27085967
- Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats:
- Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight womenS1756464617301561
- Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation.pubmed:28272338
- The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorptionS0169433216326861
- Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.pubmed:27908549
- Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathwaypubmed:27387537
- Fast and long acting neoflavonoids dalbergin isolated from Dalbergia sissoo heartwood is osteoprotective in ovariectomized model of osteoporosis: Osteoprotective …S0753332216308484
- Serum N-terminal telopeptide of type I collagen as an early marker of fracture nonunion in rabbits.pubmed:28105092
- Effects of long-term administration of pantoprazole on bone mineral density in young male rats.pubmed:27552060
- N-terminal telopeptides of type I collagen and bone mineral density for early diagnosis ofnonunion: An experimental study in rabbits.pubmed:27512225
- Micromolar Levels of Sodium Fluoride Promote Osteoblast Differentiation Through Runx2 Signalingpubmed:28083749
- Effects of fluoride on insulin signaling and bone metabolism in ovariectomized rats.pubmed:27908407
- Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation.pubmed:27987727
- Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized ratsS0753332216322752
- Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Dietarticle:10.1007
- Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken10.159:1806-9061-2016-0285
- Oral administration of kaempferol inhibits bone loss in rat model of ovariectomy-induced osteopeniapubmed:29031689
- MiR‑142‑5p promotes bone repair by maintaining osteoblast activitypubmed:27085967
- A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice.pubmed:28060768
- Biomimetic fabrication of antibacterial calcium phosphates mediated by polydopamine. pubmed:29049953
- Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation.pubmed:28742995
- A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ò-catenin Signaling Pathways in Ovariectomized RatsISSN 2249 – 622X
- Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performances10.1039/C7TB02620J
- Osteogenic commitment and differentiation of human mesenchymal stem cells by low-intensity pulsed ultrasound stimulation.pubmed:28621452
- BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect modelPubmed:30033316
- Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats10.1016:j.jff.2018.04.048
- Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat modelPubmed:29705515
- Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathwayPubmed:29883721
- Subsequent somatic axis and bone tissue metabolism responses to a low-zinc diet with or without phytase inclusion in broiler chickensPubmed:29373588
- Stabilization of Ca-deficient hydroxyapatite in biphasic calcium phosphate ceramics by adding alginate to enhance their biological performancesc7tb02620j
- Effects of constrained dynamic loading, CKIP‑1 gene knockout and combination stimulations on bone loss caused by mechanical unloadingPubmed:29956799
- A Comparison of 1‐and 3.2‐MHz Low‐Intensity Pulsed Ultrasound on Osteogenesis on Porous Titanium Alloy Scaffolds: An In Vitro and In Vivo StudyPubmed:29781183
- Dietary supplementation for Santa Inês hair ewes on pasture at pre-and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolismPubmed:29931604
- Effects of KLD-12/Recombinant Human Bone Morphogenetic Protein-2 Nanofiber Scaffold on the Osteogenic Activity of Rabbit Bone Marrow Mesenchymal Stem Cells10.1166:jbt.2018.1719
- Enhanced osteoinductivity of porous biphasic calcium phosphate ceramic beads with high content of strontium incorporated calcium deficient hydroxyapatiteDoi: 10.1039/C8TB01554F
- Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats
- Beta-Cyclodextrin Modified Mesoporous Bioactive Glass Nanoparticles/Silk Fibroin Hybrid Nanofibers as an Implantable Estradiol Delivery System for the Potential …Pubmed: 30255905
- In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem CellsDoi: 10.1016/j.carbon.2018.10.081
- The decline of whole-body glucose metabolism in ovariectomized ratsPubmed: 30292771
- Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway
- Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise
- NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblastsPubmed: 30455992
- Osteoinductivity of porous biphasic calcium phosphate ceramic spheres with nanocrystalline and their efficacy in guiding bone regenerationPubmed: 30629405
- CoCr porous scaffolds manufactured via selective laser melting in orthopedics: Topographical, mechanical, and biological characterizationPubmed: 30689288
- Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cellsPubmed: 30731080
- Effect and mechanism of psoralidin on promoting osteogenesis and inhibiting adipogenesisDoi: 10.1016/j.phymed.2019.152860
- Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and OsteoblastsPubmed: 30838809
- Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
- Macrophage inhibits the osteogenesis of fibroblasts in ultrahigh molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis
- Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed …Pubmed: 30983556
- Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats.
- Silent Mating Type Information Regulation 2 Homolog (SIRT1) Influences Osteogenic Proliferation and Differentiation of MC3T3-E1 Cells via Regulation of miR-132 …Pubmed: 30923307
- Alpha ketoglutarate exerts a pro-osteogenic effect in osteoblast cell lines through activation of JNK and mTOR/S6K1/S6 signaling pathwaysPubmed: 31051157
- Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …
- A dual functional bone‐defect‐filling material with sequential antibacterial and osteoinductive properties for infected bone defect repairPubmed: 31173657
- Use of Polyphenol Tannic Acid to Functionalize Titanium with Strontium for Enhancement of Osteoblast Differentiation and Reduction of Osteoclast ActivityPubmed: 31362449
- Laboratory Monitoring of Bone Tissue Remodeling after Augmentation of Impression Intraarticular Fracture with Different Types of Bone GraftPubmed: 31630302
- Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling PathwayPubmed: 31632013
- AAV-anti-miR-214 prevents collapse of the femoral head in osteonecrosis by regulating osteoblast and osteoclast activitiesPubmed: 31739209
- Alleviating Effect of α-Lipoic Acid and Magnesium on Cadmium-Induced Inflammatory Processes, Oxidative Stress and Bone Metabolism Disorders in Wistar RatsPubmed: 31739465
- MiR-296 Promotes Osteoblast Differentiation by Upregulating CbfalPubmed: 31694033
- Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signalsPubmed: 31826180
- Vinpocetine inhibits RANKL-induced osteoclastogenesis and attenuates ovariectomy-induced bone lossPubmed: 31846839
- BMP9 reduces bone loss in ovariectomized mice by dual regulation of bone remodelingPubmed: 31914211
- EDTA-Modified 17β-Estradiol-Laden Upconversion Nanocomposite for Bone-Targeted Hormone Replacement Therapy for OsteoporosisPubmed: 32194868
- Rutin Improves Bone Histomorphometric Values by Reduction of Osteoclastic Activity in Osteoporosis Mouse Model Induced by Bilateral OvariectomyPubmed: 32172552
- Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cellsPubmed: 32064734
- HSP90 inhibitors strengthen extracellular ATP‐stimulated synthesis of interleukin‐6 in osteoblasts: Amplification of p38 MAP kinasePubmed: 32567086
- QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolismPubmed: 32382069
- Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanismPubmed: 33000198
- Comparative study of DHA-enriched phosphatidylcholine and EPA-enriched phosphatidylcholine on ameliorating high bone turnover via regulation of the …Pubmed: 33140795
- A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration
- Research on the Mechanism of miR-142-5p's Promotion of the Function and Mineralization of Osteoblasts by Down-regulating the Expression of WWP1 Gene
- 淫羊藿水提物及提取药渣的抗骨质疏松药效比较研究
- Bioinspired polydopamine/graphene oxide/collagen nanofilms as a controlled release carrier of bioactive substances
- In vitro comparison of titanium surface conditioning via boron-compounds and sand-blasting acid-etching
- Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/¦Â©\catenin pathway33950533
- Studies of osteoblast-like MG-63 cellular proliferation and differentiation with cyclic stretching cell culture system on biomimetic hydrophilic layers modified polydimethylsiloxane substrate
- Andrographolide prevents bone loss via targeting ERRalpha‐regulated metabolic adaption of osteoclastogenesis34233019
- Effects of icariin on the fracture healing in young and old rats and its mechanism34511043
- Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway34512780
- Spatiotemporal Management of the Osteoimmunomodulation of Fibrous Scaffolds by Loading a Novel Amphiphilic NanomedicinePubmed:35311248
- Notoginsenoside R1 Promotes Migration, Adhesin, Spreading, and Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stromal CellsPubmed:35684342
- Effects of 8-week High-Intensity Interval Training and Moderate-Intensity Continuous Training on Bone Metabolism in Sedentary Young FemalesPubmed:35096081
- Effect of Antibacterial Enoxacin on the Properties of Injectable Nano-hydroxyapatite/Polyurethane Cement for Bone Repairing