Polyclonal Antibody to Peroxiredoxin 6 (PRDX6)

1-Cys; AOP2; NSGPx; PRX; aiPLA2; p29; Acidic calcium-independent phospholipase A2; Antioxidant protein 2; Non-selenium glutathione peroxidase; Red blood cells page spot 12

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 114.00 US$ 266.00 US$ 380.00 US$ 950.00 US$ 3,800.00
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Overview

Properties

Product No.PAF756Ra02
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPF756Ra02-Recombinant Peroxiredoxin 6 (PRDX6)
Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Rat Testis Tissue Primary Ab: 10µg/ml Rabbit Anti-Rat PRDX6 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat PRDX6 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Kidney Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat PRDX6 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant PRDX6, Rat.
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Cerebrum lysate; Lane3: Rat Cerebellum lysate; Lane4: Rat Spinal cord lysate
Primary Ab: 1µg/mL Rabbit Anti-Rat PRDX6 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: A549 cell lysate; Lane2: Rat Liver lysate; Lane3: Rat Lung lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Rat PRDX6 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PRDX6. It has been selected for its ability to recognize PRDX6 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

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Citations

High-mobility group box-1, promising serological biomarker for the distinction of human WNV disease severityPubmed:25197038
Differences in Proinflammatory Property of Six Subtypes of Peroxiredoxins and Anti-Inflammatory Effect of Ligustilide in Macrophagespubmed:27716839