Polyclonal Antibody to Prion Protein (PRNP)

CD230; GSS; ASCR; CJD; PRIP; PrP; PrP2730; PrPc; Major prion protein; Creutzfeldt-Jakob Disease; Gerstmann-Strausler-Scheinker Syndrome; Fatal Familial Insomnia

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 103.00 US$ 240.00 US$ 343.00 US$ 858.00 US$ 3,430.00
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Overview

Properties

Product No.PAB680Hu02
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction CD & Adhesion molecule Infection immunity Neuro science

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB680Hu01-Recombinant Prion Protein (PRNP)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Sample: Human U2OS cell; Primary Ab: 20µg/ml Rabbit Anti-Human PRNP Antibody Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)

Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Porcine Cerebrum lysate Primary Ab: 0.5µg/ml Rabbit Anti-Human PRNP Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PRNP. It has been selected for its ability to recognize PRNP in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Sus scrofa; Porcine (Pig)

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Citations

Altered levels of plasma neuron-derived exosomes and their cargo proteins characterize acute and chronic mild traumatic brain injuryPubmed: 30605353
Effects of cellular prion protein on rapid eye movement sleep deprivation-induced spatial memory impairmentPubmed: 31912703