Polyclonal Antibody to Profilin 1 (PFN1) Mus musculus (Mouse) Polyclonal antibody

Epididymis tissue protein Li 184a

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Profilin 1 (PFN1) Packages (Simulation)
  • Polyclonal Antibody to Profilin 1 (PFN1) Packages (Simulation)
  • Polyclonal Antibody to Profilin 1 (PFN1) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse PFN1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • PAC233Mu01.jpg Figure. Western Blot; Sample: Recombinant PFN1, Mouse.
  • Polyclonal Antibody to Profilin 1 (PFN1) Western Blot; Sample: Rat Liver lysate
    Primary Ab: 0.3µg/ml Rabbit Anti-Mouse PFN1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Profilin 1 (PFN1) Western Blot; Samples: Lane1: LO2 cell lysate; Lane2: Jurkat cell lysate; Lane3: HepG2 cell lysate;
    Primary Ab: 0.3µg/ml Rabbit Anti-Mouse PFN1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PFN1. It has been selected for its ability to recognize PFN1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical ImplementationPubmed:26889680
  • Comparative proteomics analysis of microvesicles in human serum for the evaluation of osteoporosisPubmed: 31081149
  • Evaluation of profilin 1 as a biomarker in myocardial infarctionPubmed: 32767339
  • Reduced perforin release from T cells as a mechanism underlying hypothermia‐mediated neuroprotection
  • ЦИркУлИрУЮщИе актИН-СвЯЗЫваЮщИе БелкИ ПрИ ПроГреССИроваНИИ рака ГортаНИ И ГортаНоГлоткИ
  • The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer
  • Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status34243097

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