Polyclonal Antibody to Programmed Cell Death Protein 1 (PD1)

CD279; PDCD1; SLEB2; HPD1P

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 95.00 US$ 221.00 US$ 316.00 US$ 790.00 US$ 3,160.00
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Overview

Properties

Product No.PAA751Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC,ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryApoptosis Immune molecule Endocrinology Autoimmunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA751Hu01-Recombinant Programmed Cell Death Protein 1 (PD1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 20ug/ml Rabbit Anti-Human PDCD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PDCD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDCD1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PDCD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Tonsil Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PDCD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Appendix Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PDCD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PDCD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human PDCD1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: PDCD1 transfected Human 293F cell lysate;
Primary Ab: 20µg/ml Mouse Anti-Human PDCD1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
FITC staining on IF;
Sample: 293F cell
Primary Ab: 20µg/ml Rabbit Anti-Human PDCD1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Sample: Rat Spleen lysate Primary Ab: 0.1µg/ml Rabbit Anti-Human PDCD1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
Western Blot; Sample: Porcine Lymph node lysate;
Primary Ab: 5µg/ml Rabbit Anti-Human PDCD1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PD1. It has been selected for its ability to recognize PD1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Increment services

Citations

Circulating soluble programmed death-1 levels may differentiate immune-tolerant phase from other phases and hepatocellular carcinoma from other clinical diseases in chronic hepatitis B virus infection.pubmed:28545019
Потенциальные биомаркеры эффективности терапии ниволумабом при метастатическом почечно-клеточном раке:
АНАЛИЗ РЕЗУЛЬТАТОВ ЛЕЧЕНИЯ БОЛЬНЫХ МЕТАСТАТИЧЕСКИМ РАКОМ ПОЧКИ, ПОЛУЧАВШИХ АНТИ-PD-1‑ТЕРАПИЮ В РАМКАХ ПРОГРАММЫ …
Design and Synthesis of A PD-1 Binding Peptide and Evaluation of Its Anti-Tumor ActivityPubmed: 30699956
Clinical significance of soluble programmed cell death-1 and soluble programmed cell death-ligand 1 in patients with locally advanced rectal cancer treated …Pubmed: 30807610
Predictive Value of Soluble PD-1, PD-L1, VEGFA, CD40 Ligand and CD44 for Nivolumab Therapy in Advanced Non-Small Cell Lung Cancer: A Case-Control StudyPubmed: 32085544