Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) Homo sapiens (Human) Polyclonal antibody

Cyclin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.38mg/ml
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  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) Packages (Simulation)
  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) Packages (Simulation)
  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) DAB staining on IHC-P;
    Samples: Human Thyroid cancer Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) DAB staining on IHC-P;
    Samples: Human Rectum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA591Hu01.jpg Western Blot; Sample: Human Liver lysate; Primary Ab: 2µg/ml Rabbit Anti-Human PCNA Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PCNA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effectsPubmed:27233913
  • Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic ratsPubmed:30026088
  • Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic ratsPubmed: 30205096
  • Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metforminPubmed: 30513216
  • Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activityPubmed: 31216504
  • The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned PigletsPubmed: 31426309
  • Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian …Pubmed: 31600920
  • Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosisPubmed: 32565124
  • Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male ratsPubmed: 33152939
  • Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats33750916
  • Local DNA synthesis is critical for DNA repair during oocyte maturation34415018
  • Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic …34859376
  • Inflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus …Pubmed:35715546
  • Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model …Pubmed:35204140
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
  • Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …

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