Polyclonal Antibody to Protein Disulfide Isomerase (PDI) Rattus norvegicus (Rat) Polyclonal antibody

P4HB; ERBA2L; PDIA1; PO4DB; PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta; Prolyl 4-Hydroxylase Subunit Beta; Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Protein Disulfide Isomerase (PDI) Packages (Simulation)
  • Polyclonal Antibody to Protein Disulfide Isomerase (PDI) Packages (Simulation)
  • Polyclonal Antibody to Protein Disulfide Isomerase (PDI) DAB staining on IHC-P; Sample: Rat Small intestine Tissue Primary Ab: 10µg/ml Rabbit Anti-Rat PDI Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Protein Disulfide Isomerase (PDI) DAB staining on IHC-P;
    Sample: Rat Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Rat PDI Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB061Ra01.jpg Western Blot; Sample: Lane1: Rat Thymus lysate; Lane2: U87MG cell lysate; Lane3: MCF7 cell lysate Primary Ab: 0.2µg/ml Rabbit Anti-Rat PDI Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PDI. It has been selected for its ability to recognize PDI in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • A Proteomic-Based Approach to Study the Mechanism of Cytotoxicity Induced by Interleukin-1α and Cycloheximidearticle:10.1007
  • Tunable activation of therapeutic platelet-rich plasma by pulse electric field: Differential effects on clot formation, growth factor release, and platelet …Pubmed: 30256831
  • CALCIUM CONTROLLED ACTIVATION OF PLATELETS VIA ELECTRICAL STIMULATION
  • Endothelial microparticle-associated protein disulfide isomerase increases platelet activation in diabetic coronary heart disease34285139

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