Polyclonal Antibody to Protein Disulfide Isomerase (PDI)

P4HB; ERBA2L; PDIA1; PO4DB; PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta; Prolyl 4-Hydroxylase Subunit Beta; Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 103.00 US$ 239.00 US$ 342.00 US$ 855.00 US$ 3,420.00
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Overview

Properties

Product No.PAB061Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB061Mu01-Recombinant Protein Disulfide Isomerase (PDI)
Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Mouse PDI Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)

WB Image
Western Blot; Sample: Lane1: Mouse Lung lysate; Lane2: Mouse Liver lysate; Lane3: Mouse Kidney lysate; Lane4: Mouse Cerebrum lysate
Primary Ab: 2µg/mL Rabbit Anti-Mouse PDI Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Mouse Liver lysate; Lane3: Mouse Cerebrum lysate; Lane4: Mouse Thymus lysate; Lane5: HepG2 cell lysate; Lane6: MCF7 cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Mouse PDI Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: A431 cell lysate; Lane3: U87MG cell lysate; Lane4: HepG2 cell lysate; Lane5: MCF7 cell lysate; Lane6: 293T cell lysate
Primary Ab: 0.1μg/ml Rabbit Anti-Mouse PDI Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PDI. It has been selected for its ability to recognize PDI in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

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Citations

A Proteomic-Based Approach to Study the Mechanism of Cytotoxicity Induced by Interleukin-1α and Cycloheximidearticle:10.1007
Tunable activation of therapeutic platelet-rich plasma by pulse electric field: Differential effects on clot formation, growth factor release, and platelet …Pubmed: 30256831
CALCIUM CONTROLLED ACTIVATION OF PLATELETS VIA ELECTRICAL STIMULATION
Endothelial microparticle-associated protein disulfide isomerase increases platelet activation in diabetic coronary heart disease34285139