Polyclonal Antibody to Protein Kinase B Alpha (PKBa) Rattus norvegicus (Rat) Polyclonal antibody

AKT1; AKT; PKB-A; Proto-oncogene c-Akt; RAC-Alpha Serine/Threonine-Protein Kinase; V-akt Murine Thymoma Viral Oncogene Homolog 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Protein Kinase B Alpha (PKBa) Packages (Simulation)
  • Polyclonal Antibody to Protein Kinase B Alpha (PKBa) Packages (Simulation)
  • Polyclonal Antibody to Protein Kinase B Alpha (PKBa) Western Blot; Samples: Lane1: Rat Cerebrum lysate; Lane2: Mouse Cerebrum lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Rat PKBa Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PKBa. It has been selected for its ability to recognize PKBa in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosisPubmed: 24188612
  • Beta-arrestin 1 is involved in the catabolic response stimulated by hyaluronan degradation in mouse chondrocytesPubmed:25673209
  • Encapsulated VEGF 121-PLA microparticles promote angiogenesis in human endometrium stromal cells33523322
  • In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the …Pubmed:35326689
  • Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network

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