KO-Validated
Polyclonal Antibody to RAR Related Orphan Receptor Alpha (RORa)

ROR1; ROR2; NR1F1; ROR3; RZRA; Nuclear receptor subfamily 1 group F member 1; Retinoid-related orphan receptor-alpha

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAD947Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryTumor immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD947Hu01-Recombinant RAR Related Orphan Receptor Alpha (RORa)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

IHC Images

Figure. Western Blot; Sample: Recombinant RORa, Human.
Western Blot; Sample: Lane1: PC3 cell lysate; Lane2: Jurkat cell lysate; Lane3: Mouse Kidney lysate
Primary Ab: 1µg/ml Rabbit Anti-Human RORa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type PC3 cell lysate;
Lane 2: RORa knockout PC3 cell lysate;
Predicted MW: 63kd
Observed MW: 54kd
Primary Ab: 1µg/ml Rabbit Anti-Human RORa Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Human Placenta lysate; Lane2: Mouse Kidney lysate; Lane3: Jurkat cell lysate; Lane4: PC3 cell lysate;
Primary Ab: 0.5µg/ml Rabbit Anti-Human RORa Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against RORa. It has been selected for its ability to recognize RORa in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

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Citations

Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancerpubmed:28421310
Global knockdown of retinoid-related orphan receptor α in mature Purkinje cells reveals aberrant cerebellar phenotypes of spinocerebellar ataxiaPubmed: 32278059

KO-Validated Polyclonal Antibody to RAR Related Orphan Receptor Alpha (RORa)