Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4) 
FRP-4; FRPHE; Frizzled protein, human endometrium
Overview
Properties
- Product No.PAF878Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategorySignal transduction
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen n/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against SFRP4. It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerancePubMed: 26041556
- Secreted Frizzled-Related Protein 4 (SFRP4) is Elevated in Patients with Diabetes MellitusPubmed:26882051
- Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesitypubmed:27768254
- Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican AmericansPubmed:29427376
- Novel marker for gestational diabetespatent:US20180059122A1
- A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profilePubmed: 30375290
- SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinomaPubmed: 30906634
- Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic …Pubmed: 31011777
- First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitusPubmed: 33206702
- SFRP4 drives invasion in gastric cancer and is an early predictor of recurrence33277667
- Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop …