Polyclonal Antibody to Semaphorin 4D (SEMA4D)

CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 106.00 US$ 247.00 US$ 353.00 US$ 883.00 US$ 3,530.00
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Overview

Properties

Product No.PAB430Mu02
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryCD & Adhesion molecule Tumor immunity Infection immunity Immune molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB430Mu02-Recombinant Semaphorin 4D (SEMA4D)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Spleen lysate Primary Ab: 0.8µg/ml Rabbit Anti-Mouse SEMA4D Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SEMA4D. It has been selected for its ability to recognize SEMA4D in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

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Citations

Circulating semaphorin-4D and plexin-B1 levels in postmenopausal women with low bone mass: the 3-month effect of zoledronic acid, denosumab or teriparatide treatmentPubmed:25395071
Genome-wide analysis identifies an african-specific variant in SEMA4D associated with body mass index.pubmed:28296344
A Revised Staging System for Waldenström's Macroglobulinemia10.1016/j.clml.2017.07.146
Extramedullary Multiple Myeloma: Clinical Features and Prognosis in Argentina10.1016/j.clml.2017.07.144
Semaphorin 4D correlates with increased bone resorption, hypercalcemia, and disease stage in newly diagnosed patients with multiple myelomaPubmed:29748532
A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasmaPubmed: 30879960
Pathological and therapeutic implications of eosinophil-derived semaphorin 4D in eosinophilic chronic rhinosinusitisPubmed: 32035658