• KO-Validated
Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) Homo sapiens (Human) Polyclonal antibody

p72-Syk; Spleen tyrosine kinase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPE275Hu01-Recombinant Spleen Tyrosine Kinase (SYK)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) Packages (Simulation)
  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) Packages (Simulation)
  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human SYK Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human SYK Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) DAB staining on IHC-P;
    Sample: Porcine Lymph node Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human SYK Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAE275Hu01.jpg Western Blot; Sample: Porcine Spleen lysate Primary Ab: 0.5µg/ml Rabbit Anti-Human SYK Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) Figure. Western Blot; Sample: Lane1: Mouse Spleen Tissue; Lane2: Rat Spinal Cord Tissue.
  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) Western Blot; Sample: Lane1: A431 cell lysate; Lane2: Raji cell lysate; Lane3: Mouse Thymus lysate; Lane4: Rat Thymus lysate
    Primary Ab: 0.5µg/ml Rabbit Anti-Human SYK Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Spleen Tyrosine Kinase (SYK) Knockout Varification:
    Lane 1: Wild-type 293T cell lysate;
    Lane 2: SYK knockout 293T cell lysate;
    Predicted MW: 69,72kd
    Observed MW: 69kd
    Primary Ab: 5µg/ml Rabbit Anti-Human SYK Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SYK. It has been selected for its ability to recognize SYK in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinasePubmed: 31949205

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