Polyclonal Antibody to Surfactant Associated Protein A2 (SPA2)

SFTPA2B; COLEC5; SP2A; SPA1 beta; SP-A2; SPAII; Collectin-5; Pulmonary Surfactant Protein A2; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 114.00 US$ 266.00 US$ 380.00 US$ 950.00 US$ 3,800.00
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Overview

Properties

Product No.PAD521Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryInfection immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD521Ra01-Recombinant Surfactant Associated Protein A2 (SPA2)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.31mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Rat Lung Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat SPA2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Small intestine Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat SPA2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Lung Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat SPA2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant SPA2, Rat.
Figure. Western Blot; Sample: Lane1: Rat Lung Tissue; Lane2: Rat Heart Tissue.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SPA2. It has been selected for its ability to recognize SPA2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

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Citations

Effects and molecular mechanisms of intrauterine infection/inflammation on lung developmentPubmed:29747649
Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD ExacerbationPubmed:29445934